| Poz | Nazwisko | Nar. | Czas | |
|---|---|---|---|---|
| 1 | Allyson Felix |  USA | 22.50 | Q |
| 2 | Susanthika Jayasinghe |  SRI | 22.55 | Q |
| 3 | Sanya Richards | USA | 22.74 | Q |
| 4 | Muriel Hurtis-Houairi |  | 22.83 | |
| 5 | Tezzhan Naimova | | 22.84 | |
| 6 | Cydonie Mothersill | | 22.86 | |
| 7 | Veronica Campbell | | 22.87 | |
| 8 | Torri Edwards | | 22.90 | |
| 9 | Kim Gevaert | | 22.91 | |
| 10 | LaShauntea Moore | | 22.93 | |
| 11 | Aleen Bailey | | 22.94 | |
| 11 | Sherry Fletcher | | 22.94 | |
| 13 | Virgil Hodge | | 23.00 | |
| 14 | LaVerne Jones | | 23.03 | |
| 15 | Roxana Díaz | | 23.06 | |
| 16 | Debbie Ferguson-McKenzie | | 23.07 | |
| 17 | Yelena Bolsun | | 23.11 | |
| 18 | Vida Anim | | 23.16 | |
| 19 | Yuliya Chermoshanskaya | | 23.21 | |
| 20 | Joice Maduaka | | 23.22 | |
| 21 | Iryna Shtanhyeyeva | | 23.23 | |
| 22 | Joice Maduaka | | 23.24 | |
| 22 | Natalia Rusakova | | 23.24 | |
| 24 | Guzel Khubbieva | | 23.25 | SB |
| 25 | Cathleen Tschirch | | 23.30 | |
| 26 | Felipa Palacios | | 23.32 | |
| 27 | Louise Ayétotché | | 23.34 | |
| 28 | Ewelina Klocek | | 23.38 | |
| 29 | Geraldine Pillay | | 23.39 | |
| 30 | Olena Chebanu | | 23.40 | SB |
| 31 | Tahesia Harrigan | | 23.46 | |
| 32 | Zudikey Rodriguez | | 23.96 |
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