| Poz | Nazwisko | Nar. | Czas | |
|---|---|---|---|---|
| 1 | Anastasios Gousis |  GRE | 20.11 | Q |
| 2 | Usain Bolt |  JAM | 20.12 | Q |
| 3 | Brendan Christian |  ANT | 20.23 | Q |
| 4 | Marcin Jedrusinski |  POL | 20.31 | Q |
| 4 | Arnaldo Abrantes |  POR | 20.31 | q |
| 4 | Bryan Barnett |  CAN | 20.31 | Q |
| 7 | Marvin ANDERSON | JAM | 20.43 | Q |
| 8 | Kristof Beyens |  BEL | 20.44 | Q |
| 8 | Rodney Martin |  USA | 20.44 | Q |
| 10 | Wallace Spearmon | USA | 20.45 | Q |
| 11 | Paul Hession |  IRL | 20.46 | Q |
| 11 | Tyson Gay | USA | 20.46 | Q |
| 13 | Shingo Suetsugu |  JPN | 20.47 | Q |
| 13 | Churandy Martina |  NED | 20.47 | Q |
| 13 | Sandro Viana |  BRA | 20.47 | Q |
| 16 | Brian Dzingai |  ZIM | 20.50 | Q |
| 17 | Matic Osovnikar |  SLO | 20.51 | Q |
| 18 | Visa Hongisto |  FIN | 20.56 | q |
| 19 | Guus Hoogmoed | NED | 20.57 | q |
| 20 | Francis Obikwelu | POR | 20.61 | Q |
| 21 | Amr Ibrahim Mostafa SEOUD |  EGY | 20.65 | q |
| 21 | James DOLPHIN |  NZL | 20.65 | q |
| 23 | Marco CRIBARI |  SUI | 20.71 | q |
| 24 | Marc Schneerberger | SUI | 20.72 | Q |
| 25 | Tomoya KAMIYAMA | JPN | 20.78 | Q |
| 26 | Ivan Teplykh |  RUS | 20.81 | q |
| 26 | Jacobi MITCHELL |  BAH | 20.81 | q |
| 28 | Shinji Takahira | JPN | 20.83 | Q |
| 29 | Christopher Williams | JAM | 20.88 | Q |
| 30 | Basilio DE MORAES | BRA | 21.09 | Q |
| 31 | Franklin NAZARENO |  ECU | 21.18 | Q |
| 32 | Patrick Johnson |  AUS | 21.48 | Q |
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